Microscopy

Colocalization of fluorescence and raman microscopic images for training data collection in matlab

The following Matlab project contains the source code and Matlab examples used for colocalization of fluorescence and raman microscopic images for training data collection . This colocalization scheme unveils statistically significant overlapping regions by identifying correlation between fluorescence color channels and clusters from unsupervised machine learning methods like hierarchical cluster analysis (HCA) performed on Raman or CARS spectral images.

Coloc3d in matlab

The following Matlab project contains the source code and Matlab examples used for coloc3d. This script allows the user to load 3-D TIFF images, such as those derived from confocal or 2-photon microscopy, into the MATLAB workspace for analysis of colocalization between two images in 3-D.

Widefield fluorescence microscope point spread function in matlab

The following Matlab project contains the source code and Matlab examples used for widefield fluorescence microscope point spread function. This function generate the point-spread function for a widefield microscope using a scalar diffraction-limited model (Stokseth refer to [1] and [3] below) ---------------- Input parameters: lambdaEx: Excitation Wavelength (in nm) lambdaEm: Emission wavelegnth (in nm) numAper: Numerical aperture of the objective magObj: Objective total magnification rindexObj: Refractive index of the objective immersion medium

Gui for displaying image stacks, e.g. time-resolved or z-stacked microscopy images. in matlab

The following Matlab project contains the source code and Matlab examples used for gui for displaying image stacks, e.g. time-resolved or z-stacked microscopy images. . GUI for displaying image stacks, either from a 3D array given as indata or from a tiff, lsm, or other stack chosen by the user.

Filter noise and interpolate microscopy images in frequency domain in matlab

The following Matlab project contains the source code and Matlab examples used for filter noise and interpolate microscopy images in frequency domain. Intensities recorded by the microscopes (any imaging system with finite aperture) are subject to diffraction limit - the recorded features cannot be finer than the optical cutoff of the system.

Get training data to classify organelles in matlab

The following Matlab project contains the source code and Matlab examples used for get training data to classify organelles. This colocalization scheme unveils statistically significant overlapping regions by identifying correlation between fluorescence color channels and clusters from unsupervised machine learning methods like hierarchical cluster analysis (HCA) performed on Raman or CARS spectral images.

Improved blood velocity measurements with a hybrid image filtering and iterative radon transform in matlab

The following Matlab project contains the source code and Matlab examples used for improved blood velocity measurements with a hybrid image filtering and iterative radon transform . Red blood cells (RBCs) in plasma labeled with a fluorescent dye present as black disks on microscopy.
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